CD137 Antibody / 4-1BB / TNFRSF9

• Catalog Number: orb699703
• Category: Antibodies
• Description: CD137 belongs to the tumor necrosis factor receptor family and delivers a costimulatory signal to T lymphocytes. It is expressed on activated T cells and binds an inducible ligand that is found on B cells, macrophages and dendritic cells. Interactions between CD137 and its ligand are involved in antigen presentation and the generation of cytotoxic T cells. CD137 antibody may improve cancer treatment, and has been implicated in breast cancer, melanoma and lymphoma.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: r4-1BB/4603
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A portion of amino acids 19-188 from the human protein was used as the immunogen for the recombinant CD137 antibody.
• Dilution range: Immunohistochemistry (FFPE): 2-4ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant CD137 antibody is available for research use only.
• UniProt ID: Q07011
• Storage: Store the recombinant CD137 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant CD137 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human kidney with recombinant CD137 antibody (clone r4-1BB/4603). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CD137 antibody (clone r4-1BB/4603) as confirmation of integrity and purity.