CD137 Antibody / 4-1BB / TNFRSF9

• Catalog Number: orb607142
• Category: Antibodies
• Description: CD137 belongs to the tumor necrosis factor receptor family and delivers a costimulatory signal to T lymphocytes. It is expressed on activated T cells and binds an inducible ligand that is found on B cells, macrophages and dendritic cells. Interactions between CD137 and its ligand are involved in antigen presentation and the generation of cytotoxic T cells. CD137 antibody may improve cancer treatment, and has been implicated in breast cancer, melanoma and lymphoma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4-1BB/3201
• Tested applications: ELISA, FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant human CD137 protein fragment within amino acids 19-188 was used as the immunogen for the CD137 antibody.
• Dilution range: ELISA (order BSA/sodium azide-free format for coating),Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD137 antibody is available for research use only.
• UniProt ID: Q07011
• Storage: Store the CD137 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD137 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of MeOH-fixed human HEK293 cells with CD137 antibody (green, clone 4-1BB/3201) and Reddot nuclear stain (red).

IHC staining of FFPE human spleen with CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human cerebellum with CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human renal cell carcinoma with CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human renal cell carcinoma with CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Flow cytometry testing of MeOH-fixed human HEK293 cells with CD137 antibody (clone 4-1BB/3201); Red=isotype control, Blue= CD137 mAb.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CD137 antibody (clone 4-1BB/3201). These results demonstrate the foremost specificity of the 4-1BB/3201 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Western blot testing of human kidney lysate with CD137 antibody. Expected molecular weight: ~30 kDa monomer and 55-60 kDa dimer. Higher molecular weights may be observed due to glycosylation.