CD133 Rabbit Polyclonal Antibody

• Catalog Number: orb394013
• Category: Antibodies
• Description: CD133 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Drosophila, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD133 (751-848/865aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 120 kDa
• Target: PROM1
• UniProt ID: O43490
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AC133; Antigen AC133; Hematopoietic stem cell anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Hep G2 (blue). Primary Antibody (green line): Rabbit Anti-CD133 antibody (orb394013), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ethanol Overnight at 4°C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: SP2/0 (mouse) cell Lysate at 40 ug, Uterus (mouse) Lysate at 40 ug, Lovo (human) cell Lysate at 40 ug, Primary: Anti-CD133 (orb394013) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 95kD, Observed band size: 115 kD.