CD11c Antibody / ITGAX

• Catalog Number: orb749613
• Category: Antibodies
• Description: Recognizes a protein of 145kDa, identified as CD11c. CD11c (ITGAX), a member of the leukointegrin family, shares the same beta subunit with other members of the leukocyte adhesion molecule family, which includes CD11a (LFA-1), CD11b (MAC-1) and CD11d (ITGAD), but has a unique alpha chain. CD11c has been shown to play a role in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T-cell proliferation by Langerhans cells. CD11c is expressed prominently on the plasma membranes of monocytes, tissue macrophages, NK cells, and most dendritic cells (DCs). A lower level of expression is also observed on neutrophils as a result of its high level of expression on most DCs. An antibody to CD11c may aid in identification of lesions with histiocytic origin. It may also been used as a marker for hairy cell leukemia in paraffin-embedded tissues.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ITGAX/1243
• Tested applications: ELISA
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A partial recombinant protein corresponding to amino acids 637-827 from human ITGAX/CD11c was used as the immunogen for the CD11c antibody.
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD11c antibody is available for research use only.
• UniProt ID: P20702
• Storage: Store the CD1°C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD11c antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human prostate carcinoma with CD11c antibody (clone ITGAX/1243). Note staining of cancer cells. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.

IHC testing of FFPE human lung carcinoma with CD11c antibody (clone ITGAX/1243). Note staining of cancer cells. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.

IHC testing of FFPE human follicular lymphoma with CD11c antibody (clone ITGAX/1243). Note staining of cancer cells. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free CD11c Antibody (clone ITGAX/1243). Confirmation of Integrity and Purity of the Antibody.