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Catalog Number | orb19554 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to CD11b |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC |
Reactivity | Human |
Dilution range | ELISA: 1:32000, IHC-P: 10 μg/ml |
Conjugation | Unconjugated |
MW | 127 |
Target | ITGAM / CD11B |
Entrez | 3684 |
Protein Sequence | RNDGEDSYRTQ |
RRID | AB_10749632 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti Antigen CD11b p170 antibody, anti CD11 antige Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 170kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 127kDa according to NP_000623.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources. Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19554 Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
orb19554 (2µg/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Immunofluorescence analysis of U937 cells using CD11b antibody.
orb19554 Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
orb19554 (8 µg/mL) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
orb19554 Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
FC, IHC-P | |
Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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