CD10 Antibody / Neprilysin

• Catalog Number: orb534168
• Category: Antibodies
• Description: Neprilysin, also known as membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), cluster of differentiation 10 (CD10), and common acute lymphoblastic leukemia antigen (CALLA) is an enzyme that in humans is encoded by the MME gene. CD10 is a zinc-dependent metalloprotease that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. It also degrades the amyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. Synthesized as a membrane-bound protein, the neprilysin ectodomain is released into the extracellular domain after it has been transported from the Golgi apparatus to the cell surface.CD10 is expressed in a wide variety of tissues and is particularly abundant in kidney. It is also a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. [Wiki]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CDLA10-1
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: A portion of amino acids 297-483 from the human protein was used as the immunogen for the CD10 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD10 antibody is available for research use only.
• UniProt ID: P08473
• Storage: Store the CD10 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The stated application concentrations are suggested starting points. Titration of the CD10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human renal cell carcinoma with CD10 antibody (clone CDLA10-1). Required HEIR: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.

Western blot testing of human kidney lysate with CD10 antibody (clone CDLA10-1). Routinely visualized at ~100 kDa.