CCT2 Antibody

• Catalog Number: orb1256942
• Category: Antibodies
• Description: CCT2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 206-535 of human CCT2 (NP_006422.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 57kDa
• Target: CCT2
• UniProt ID: P78371
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: CCT2, 99D8.1, CCT-beta, CCTB, PRO1633, TCP-1-beta,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CCT2 antibody (orb1256942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunofluorescence analysis of HeLa cells using CCT2 antibody (orb1256942). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of A-549 cells, using 3 ug CCT2 antibody (orb1256942). Western blot was performed from the immunoprecipitate using CCT2 antibody (orb1256942) at a dilution of 1:1000.