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Catalog Number | orb1671598 |
---|---|
Category | Antibodies |
Description | CCR5 Antibody |
Clonality | Recombinant |
Clone Number | V14/2 |
Tested applications | ELISA, FC, IF, IP, WB |
Reactivity | Human |
Isotype | IgG kappa |
Immunogen | This antibody was raised by immunizing mice with a synthetic phospho peptide corresponding to the C-Terminal region of human CCR5 conjugated to BSA. |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | CCR5 |
UniProt ID | P51681 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | CD195, C-C chemokine receptor type 5, C-C CKR-5, C Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometry using the anti-CCR5 (phosphoserine 349) antibody E11/19. U937 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r1 version of E11/19 (orb1671598 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of fixed U937 cells with anti-CCR5 (phosphoserine 337) antibody V14/2. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric r version of V14/2 (orb1671598) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671598- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Western Blot using anti-CCR5 (phosphoserine 337) antibody V14/2. K562 cell lysate (35 ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of V14/2 (orb1671598) at 2 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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