CCL5 Antibody

• Catalog Number: orb1272243
• Category: Antibodies
• Description: CCL5 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5B3-D5
• Tested applications: ELISA, NeA, WB
• Reactivity: Human
• Isotype: RANTES antibody was derived form BALB/c mice and belongs to the IgG2b isotype.
• Immunogen: Monoclonal antibody against human RANTES designated as VL-1 was purified from ascites fluid on a Protein A affinity column.
• Concentration: batch dependent
• Dilution range: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of h-RANTES (100ng/mL), a concentration of 5-7 μg/mL of this antibody is required. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human RANTES when used with our biotinylated antigen affinity purified anti-human RANTES (XP-5257Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot:To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: CCL5
• UniProt ID: P13501
• NCBI: P13501
• Storage: RANTES lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
• Alternative names: Regulated upon activation normally T expressed and
• Note: For research use only
• Application notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of h-RANTES (100ng/mL), a concentration of 5-7 μg/mL of this antibody is required. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human RANTES when used with our biotinylated antigen affinity purified anti-human RANTES (XP-5257Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot:To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 citrate buffer is recommended. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 citrate buffer is recommended. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 citrate buffer is recommended. Optimal concentrations and conditions may vary.