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Catalog Number | orb1671455 |
---|---|
Category | Antibodies |
Description | Ccl11 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Mouse |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mEotaxin. Anti-Murine Eotaxin specific antibody was purified by affinity chromatography employing immobilized mEotaxin matrix. |
Concentration | batch dependent |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | Ccl11 |
UniProt ID | P48298 |
NCBI | P48298 |
Storage | Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | CCL11 Read more... |
Note | For research use only |
Application notes | Sandwich ELISA: To detect mEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biorbyt’s Biotinylated Anti-Murine Eotaxin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin. Western Blot To detect mEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect mEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Eotaxin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin.
To detect mEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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