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Catalog Number | orb1239834 |
---|---|
Category | Tools |
Description | CCL11 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, WB |
Isotype | IgG |
Immunogen | Eotaxin antibody was raised in rabbits against a peptide corresponding to amino acids near the carboxy terminus of human eotaxin.The immunogen is located within the last 50 amino acids of Eotaxin. |
Concentration | 1 mg/ml |
Dilution range | Eotaxin antibody can be used for detection of eotaxin by Western blot at 1 μg/mL. The recombinant protein can be used as positive control and monomer and homodimer are detected at approximate 9 and 18 kDa. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 9 - 18 kDa |
Target | CCL11 |
UniProt ID | P51671 |
NCBI | CAB07027 |
Storage | Eotaxin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Eotaxin Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | Eotaxin Antibody: SCYA11, SCYA11, Eotaxin, C-C mot Read more... |
Note | For research use only |
Application notes | Eotaxin antibody can be used for detection of eotaxin by Western blot at 1 μg/mL. The recombinant protein can be used as positive control and monomer and homodimer are detected at approximate 9 and 18 kDa. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of eotaxin in HeLa cell lysate containing 10, 2.5, or 1 ng of full length recombinant eotaxin with eotaxin antibody at 1 µg/mL.
Immunocytochemistry of Eotaxin in 293 cells with Eotaxin antibody at 5 µg/mL.
Immunofluorescence of Eotaxin in 293 cells with Eotaxin antibody at 10 µg/mL.
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