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Item 1 of 5
CBS Antibody (N-term)
Catalog Number: orb1929686
| Catalog Number | orb1929686 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB22961 |
| Tested applications | FC, IF, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 60587 Da |
| Target | This CBS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the N-terminal region of human CBS. |
| UniProt ID | P35520 |
| NCBI | NP_000062.1, NP_001171479.1, NP_001171480.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Cystathionine beta-synthase, Beta-thionase, Serine Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
CBS Antibody (N-term) flow cytometric analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of CBS Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
CBS Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CBS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of CBS Antibody (N-term) in mouse kidney tissue and Ramos cell line lysates (35 ug/lane). CBS (arrow) was detected using the purified Pab.
Western blot analysis of CBS (arrow) using rabbit polyclonal CBS Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CBS gene.
