Cbl-c antibody

• Catalog Number: orb345519
• Category: Antibodies
• Description: Cbl-c antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal portion of Human Cbl-c.
• Concentration: 1.2 mg/ml
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:3,000, IP: User Optimized, WB: 1:500 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human Cbl-c protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with Cbl-c from human and chimpanzee sources. Expect partial reactivity against mouse and rat sources of Cbl-c as ~83% sequence homology is on record for the immunogen sequence. Reactivity with Cbl-c from other sources has not been determined. No reactivity is expected with Cbl-a or Cbl-b.
• Conjugation: Unconjugated
• UniProt ID: Q9ULV8
• NCBI: 125987803
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Cbl-c Antibody, E3 ubiquitin-protein l
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, immunoprecipitation and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~52 kDa in size corresponding to Cbl-c by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Biorbyt's Affinity Purified anti-Cbl-c antibody shows strong nuclear and cytoplasmic staining of cells in tubuli in human kidney tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.

Biorbyt's affinity purified anti-Cbl-c antibody was used at 5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular positive staining of human pancreatic acinar epithelium at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Immunoprecipitation (right) and western blot (left) using Biorbyt's Affinity Purified anti-Cbl-c antibody shows detection of a predominant band at ~52 kDa corresponding to Cbl-c. Lysates used are from HEK293T cells transfected with empty vector or with Cbl-c and western blotting (left panel). The predicted size of Cbl-c is 52 kDa. Size markers in kDa are shown to the left of the panel. The (right panel) shows immunoprecipitation with Rabbit anti-Cbl-c followed by western blotting using a Goat anti-Cbl-c antibody.