CAT antibody

• Catalog Number: orb19252
• Category: Antibodies
• Description: Goat polyclonal antibody to CAT
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse
• Dilution range: ELISA: 1:8000, WB: 0.1-0.3 μg/ml
• Conjugation: Unconjugated
• MW: 59.8
• Target: Catalase / CAT
• Entrez: 847
• Protein Sequence: DKYNAEKPKNAIHT
• RRID: AB_10753324
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CAT antibody, anti catalase antibody, anti HG
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 2-4ug/ml.WB: Approx 60kDa band observed in Human Kidney and Liver lysates (calculated MW of 59.8kDa according to NP_001743.1). Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

References

Patche, Jessica et al. Diabetes-induced hepatic oxidative stress: a new pathogenic role for glycated albumin Free Radic Biol Med, 102, 133-148 (2017)

PubMed: 27890722

Reactivity

Mouse

Immunohistochemical staining of Human Spleen using CAT antibody

Western blot anlaysis of Human Kidney lysate using CAT antibody

Immunohistochemical staining of Human Liver using CAT antibody

Western blot analysis of Human Kidney (A) and Liver (B) and Mouse Kidney (C) and Liver (D) lysates using CAT antibody