Caspase 8 Rabbit Polyclonal Antibody

• Catalog Number: orb10241
• Category: Antibodies
• Description: Caspase 8 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Mouse, Porcine
• Reactivity: Human, Rat, Sheep
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from rat Caspase-8 subunit p10 (411-482/482aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 12/55 kDa
• Target: CASP8
• UniProt ID: Q9JHX4
• RRID: AB_10747552
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Caspase-8 p10; Caspase-8 subunit p10; ALPS2B; Amyo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Barton, Samantha K. et al. Differential short-term regional effects of early high dose erythropoietin on white matter in preterm lambs after mechanical ventilation J Physiol, 594, 1437-1449 (2016)

PubMed: 26332509

Blank control (blue line): U251 (blue). Primary Antibody (green line): Rabbit Anti-caspase-8 antibody (orb10241), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/test. Protocol, The cells were fixed with 70% ethanol overnight at 4℃ and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Caspase 8) Polyclonal Antibody, Unconjugated (orb10241) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Jurkat (Human) Cell Lysate at 30 ug, K562 (Human) Cell Lysate at 30 ug, U87MG (Human) Cell Lysate at 30 ug, Primary: Anti-caspase-8 subunit p18 (orb10241) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55/18 kD, Observed band size: 57 kD.

Tissue/cell: Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Caspase 8) polyclonal Antibody, Unconjugated (orb10241) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.