Caspase 8 Mouse Monoclonal Antibody

• Catalog Number: orb500937
• Category: Antibodies
• Description: Caspase 8 Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4G12
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant human Caspase 8 Protein
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:2000-5000, IHC-P=1:400-1000, IHC-F=1:500-1000, IF=1:500-1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 43 kDa
• Target: CASP8
• UniProt ID: Q14790
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Caspase-8 subunit p10; ALPS2B; Amyotrophic lateral
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (orb500937) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of orb500937 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of orb500937 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of orb500937 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of orb500937 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Sample: 293T-UV (Human) Cell Lysate at 30 ug, U251-UV (Human) Cell Lysate at 30 ug, Primary: Anti-Caspase 8 (orb500937) at 1/4000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 41'43'55 kD, Observed band size: 43 kD.

Sample: Hela (Human) Cell Lysate at 30 ug Jurkat (Human) Cell Lysate at 30 ug Molt-4 (Human) Cell Lysate at 30 ug K562 (Human) Cell Lysate at 30 ug Primary: Anti-Caspase 8 (orb500937) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41'43'55 kD Observed band size: 43 kD.

Sample: Stomach (Mouse) Lysate at 40 ug, Spleen (Mouse) Lysate at 40 ug, Lymph node (Mouse) Lysate at 40 ug, Urinary bladder (Mouse) Lysate at 40 ug, Primary: Anti-Caspase 8 (orb500937) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41'43'55 kD, Observed band size: 55'53D.