Caspase-8 Cleaved Antibody

• Catalog Number: orb1294354
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Caspase 8 cleaved
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Dilution range: Western Blot 1:500 - 1:2000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:200 – 1:300
• Conjugation: Unconjugated
• Target: Caspase-8 Cleaved
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Caspase 8 antibody - at 1/1000 dilution. Lysates at 50 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294354 overnight at 4°C.

Caspase 8 antibody - at 1/1000 dilution, Lysates at 50 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294354 overnight at 4°C.

immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Caspase 8 with orb1294354 at 1/200.

Customer feedback Image. Immunohistochemical of Paraffin-embedded. Sample: Human colon cancer. Caspase 8 antibody (orb1294354): 1-200.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294354 Anti-Caspase 8 antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.