Caspase-3 (P10)/CASP3 Antibody

• Catalog Number: orb137849
• Category: Antibodies
• Description: Caspase-3 (P10)/CASP3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-3 (P10) (220-236aa CAMLKQYADKLEFMHIL).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 31608 MW
• UniProt ID: P42574
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Caspase-3;CASP-3;3.4.22.56;Apopain;Cysteine protea
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Lung Cancer Tissue using Caspase-3 (P10) antibody

Western blot analysis of Lane 1: HELA Cell Lysate, Lane 2: SMMC Cell Lysate using Caspase-3 (P10) antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Intestinal Cancer Tissue using Caspase-3 (P10) antibody