CASP3 Antibody

• Catalog Number: orb1258872
• Category: Antibodies
• Description: CASP3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein of human CASP3.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 31kDa
• Target: CASP3
• UniProt ID: P42574
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Caspase-3 Antibody: CPP32, SCA-1, CPP32B, CPP32, C
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CASP3 antibody (orb1258872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human mammary cancer using Caspase 3 antibody (orb1258872) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using Caspase 3 antibody (orb1258872) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using Caspase 3 antibody (orb1258872) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using Caspase 3 antibody (orb1258872) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of C6 cells using Caspase 3 antibody (orb1258872) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Caspase 3 antibody (orb1258872) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2OS cells using Caspase 3 antibody (orb1258872) at dilution of 1:100. Blue: DAPI for nuclear staining.