CASP1 Antibody

• Catalog Number: orb1261749
• Category: Antibodies
• Description: CASP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-311 of human Caspase 1 (NP_150635.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 30kDa/48kDa
• Target: CASP1
• UniProt ID: P29466
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Caspase-1 Antibody: ICE, P45, IL1BC, IL1BCE, Caspa
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Caspase 1 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human liver cancer using Caspase 1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using Caspase 1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using Caspase 1 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of Raw264. 7 cells using Caspase 1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of THP-1 cells, using 3 ug Caspase 1 antibody. Western blot was performed from the immunoprecipitate using Caspase 1 antibody at a dilution of 1:1000.