Cas 9 antibody

• Catalog Number: orb420317
• Category: Antibodies
• Description: Cas 9 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, Multiplex Assay, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:25,000-1:30,000, IF: 1µg/mL, WB: 1µg/mL
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
• Conjugation: Unconjugated
• UniProt ID: Q99ZW2
• NCBI: NP_269215.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, C
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, Immunofluorescence, and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence of Rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+ Cas9 or GFP+ CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1 µg/mL. Secondary: Donkey anti-Rabbit at 1 µg/mL, staining seen in red. Bottom images show GFP stain overlay.

Western Blot of Rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15 µg per lane. Primary Antibody cas9 used at 1 µg/mL using orb348637 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40000 for 30 min at room temp. Expect: 158 kDa.

Western Blot results of Rabbit Anti-Cas 9 Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: HEK/293 Cas9 Over Expressing WCL. Lane 3: HEK/293 non Transfected WCL. Load: 10ul. Primary Antibody: Rabbit Anti-Cas 9 Antibody at 1 µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n orb347654) at 1:70000 for 30 min at RT. Blocking: BlockOut (p/n orb348644) for 30 min at RT.