Cas 9 antibody

• Catalog Number: orb420316
• Category: Antibodies
• Description: Cas 9 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, Multiplex Assay, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:25,000-1:30,000, IF: 1µg/mL, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
• Conjugation: Unconjugated
• UniProt ID: Q99ZW2
• NCBI: NP_269215.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, C
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, Immunofluorescence, and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence of Rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+ Cas9 or GFP+ CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1 µg/mL. Secondary: Donkey anti-Rabbit at 1 µg/mL, staining seen in red. Bottom images show GFP stain overlay.

Western Blot of Rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15 µg per lane. Primary Antibody cas9 used at 1 µg/mL using orb348637 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40000 for 30 min at room temp. Expect: 158 kDa.

Western Blot results of Rabbit Anti-Cas 9 Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: HEK/293 Cas9 Over Expressing WCL. Lane 3: HEK/293 non Transfected WCL. Load: 10ul. Primary Antibody: Rabbit Anti-Cas 9 Antibody at 1 µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n orb347654) at 1:70000 for 30 min at RT. Blocking: BlockOut (p/n orb348644) for 30 min at RT.