CARD11 Antibody

• Catalog Number: orb1247706
• Category: Antibodies
• Description: CARD11 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QRKTIWVDEDQL
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 150+65kDa bands observed in lysates of cell line Jurkat (calculated MW of 133kDa according to NP_115791.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:In paraffin embedded Human Spleen shows nuclear staining. Recommended concentration: 2-5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CARD11
• UniProt ID: Q9BXL7
• NCBI: NP_115791.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CARD11, caspase recruitment domain family, member
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 150+65kDa bands observed in lysates of cell line Jurkat (calculated MW of 133kDa according to NP_115791.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:In paraffin embedded Human Spleen shows nuclear staining. Recommended concentration: 2-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247706 staining (1 ug/ml) of Human Jurkat cells (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

orb1247706 (2 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.