Car(Carnosine) ELISA Kit

• Catalog Number: orb1806545
• Category: Assays and Kits
• Description: Car(Carnosine) ELISA Kit
• Reactivity: All
• Range: 14.07-900 ng/mL
• Target: Car
• Sensitivity: 3.53 ng/mL
• Alternative names: β-Alanyl-L-Histidine
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Carnosine(Car) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carnosine(Car). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carnosine(Car) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.