Carboxypeptidase B antibody (Peroxidase)

• Catalog Number: orb344722
• Category: Antibodies
• Description: Carboxypeptidase B antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Porcine
• Isotype: IgG
• Immunogen: Carboxypeptidase B [Porcine Pancreas]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:5,000, WB: 1:250 - 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Carboxypeptidase B Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas]. Cross reactivity against Carboxypeptidase B from other tissues and species is unknown.
• Conjugation: HRP
• UniProt ID: P09955
• NCBI: NP_999334.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Carboxypeptidase B antibody HRP conjug
• Note: For research use only
• Application notes: Anti-Carboxypeptidase B peroxidase Antibody has been tested in western blot and suitable to be assayed against 1.0 ug of Carboxypeptidase B [Porcine Pancreas] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for Anti-Carboxypeptidase B Antibody.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Carboxypeptidase B Antibody Peroxidase Conjugated. Lane 1: Carboxypeptidase B. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carboxypeptidase B Antibody Peroxidase Conjugated at 1:1000 overnight at 4°C. Secondary antibody: n/a. Block: orb348637 for 30 minutes at RT. Predicted/Observed size: 47 kDa, observed at 35 kDa for Carboxypeptidase B. Expected molecular weight for processed protein is ~34.7 kDa.