Carboxypeptidase B antibody (Biotin)

• Catalog Number: orb344750
• Category: Antibodies
• Description: Carboxypeptidase B antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Porcine
• Isotype: IgG
• Immunogen: Carboxypeptidase B [Porcine Pancreas]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-CARBOXYPEPTIDASE B (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas]. Cross reactivity against Carboxypeptidase B from other sources may occur but have not been specifically determined.
• Conjugation: Biotin
• UniProt ID: P09955
• NCBI: NP_999334.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Carboxypeptidase B antibody biotin con
• Note: For research use only
• Application notes: Anti-CARBOXYPEPTIDASE B Biotin Antibody has been tested by Western Blot and is suitable to be assayed against 1.0 ug of Carboxypeptidase B in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Carboxypeptidase B antibody Biotin conjugated. Lane 1: Carboxypeptidase B. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carboxypeptidase B antibody Biotin conjugated at 1:1000 overnight at 4°C. Secondary antibody: HRP conjugated rabbit secondary antibody at 1:40000 for 30 min at RT. Block: Blocking buffer for Fluorescent Western Blotting (orb348637) at RT for 30 min. Predicted/Observed size: ~35 kDa.