Carboxypeptidase B antibody (Biotin)

• Catalog Number: orb344749
• Category: Antibodies
• Description: Carboxypeptidase B antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Porcine
• Isotype: IgG
• Immunogen: Carboxypeptidase B [Porcine Pancreas]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
• Form/Appearance: Lyophilized
• Purity: Anti-CARBOXYPEPTIDASE B (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas]. Cross reactivity against Carboxypeptidase B from other sources may occur but have not been specifically determined.
• Conjugation: Biotin
• UniProt ID: P09955
• NCBI: NP_999334.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Carboxypeptidase B antibody biotin con
• Note: For research use only
• Application notes: Anti-CARBOXYPEPTIDASE B Biotin Antibody has been tested by Western Blot and is suitable to be assayed against 1.0 ug of Carboxypeptidase B in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Carboxypeptidase B antibody Biotin conjugated. Lane 1: Carboxypeptidase B. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carboxypeptidase B antibody Biotin conjugated at 1:1000 overnight at 4°C. Secondary antibody: HRP conjugated rabbit secondary antibody at 1:40000 for 30 min at RT. Block: Blocking buffer for Fluorescent Western Blotting (orb348637) at RT for 30 min. Predicted/Observed size: ~35 kDa.