You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb344592 |
---|---|
Category | Antibodies |
Description | Carbonic Anhydrase II antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Carbonic Anhydrase II Antibody was produced by repeated immunizations with human erythrocytes Carbonic Anhydrase II. |
Concentration | 1.1 mg/mL |
Dilution range | ELISA: 1:165,000, IHC: User Optimized, WB: 1:2000 to 1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-Carbonic Anhydrase II Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes]. Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined. |
Conjugation | Unconjugated |
UniProt ID | P00918 |
NCBI | NP_000058.1 |
Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Carbonic Anhydrase II Antibody, Carbon Read more... |
Note | For research use only |
Application notes | Carbonic Anhydrase II Antibody has been tested by ELISA, western blot, and immunohistochemistry. Expect ~29.2kDa. Researchers should determine optimal titers for applications that are not stated below. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry with anti-carbonic anhydrase II antibody showing carbonic anhydrase II staining in nucleus and cytoplasm of proximal and distal tubules, bowman's capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3'3-diamino-benzidine substrate (DAB) or WARP RED.
Western Blot of Rabbit Anti-Carbonic Anhydrase II primary antibody. Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase II primary antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Blocking: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II. Other band(s): None.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Bovine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating