Carbonic Anhydrase II antibody

• Catalog Number: orb344591
• Category: Antibodies
• Description: Carbonic Anhydrase II antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Carbonic Anhydrase II Antibody was produced by repeated immunizations with human erythrocytes Carbonic Anhydrase II.
• Concentration: 1.1mg/mL
• Dilution range: ELISA: 1:165,000, IHC: User Optimized, WB: 1:2000 to 1:10,000
• Form/Appearance: Lyophilized
• Purity: Anti-Carbonic Anhydrase II Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes]. Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: P00918
• NCBI: NP_000058.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Carbonic Anhydrase II Antibody, Carbon
• Note: For research use only
• Application notes: Carbonic Anhydrase II Antibody has been tested by ELISA, western blot, and immunohistochemistry. Expect ~29.2kDa. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry with anti-carbonic anhydrase II antibody showing carbonic anhydrase II staining in nucleus and cytoplasm of proximal and distal tubules, bowman's capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3'3-diamino-benzidine substrate (DAB) or WARP RED.

Western Blot of Rabbit Anti-Carbonic Anhydrase II primary antibody. Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase II primary antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Blocking: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II. Other band(s): None.