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Catalog Number | orb344335 |
---|---|
Category | Antibodies |
Description | Carbonic Anhydrase I antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Carbonic Anhydrase I [Human Erythrocytes] |
Concentration | 1.0 mg/ml |
Dilution range | ELISA: 1:2,000 - 1:20,000, IHC: 10µg/ml, WB: 1:500 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-CARBONIC ANHYDRASE I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes]. Cross reactivity against Carbonic Anhydrase I from other tissues and species may occur but have not been specifically determined. |
Conjugation | Unconjugated |
UniProt ID | P00915 |
NCBI | NP_001122301.1 |
Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | goat anti-Carbonic Anhydrase I Antibody, Car1 anti Read more... |
Note | For research use only |
Application notes | Anti-Carbonic Anhydrase 1 has been tested in western blot and immunohistochemistry and is suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry of Goat anti-Carbonic Anhydrase I antibody. Tissue: human spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Carbonic Anhydrase I antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10000 for 45 min at RT. Staining: Carbonic Anhydrase 1 as precipitated red signal with hematoxylin purple nuclear counterstain.
Western Blot of Goat anti-Carbonic Anhydrase I Antibody. Lane 1: Carbonic Anhydrase I. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase I antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP goat secondary antibody (p/n orb346990) at 1:40000 for 30 min at RT. Block: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase I.
FC, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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