Carbonic Anhydrase 9 antibody

• Catalog Number: orb323113
• Category: Antibodies
• Description: Mouse monoclonal antibody to Carbonic Anhydrase 9
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, IP, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Carbonic Anhydrase 9. The exact sequence is proprietary.
• Dilution range: WB: 1:1000-1:3000, IHC-P: 1:100-1:200, IP: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CA9
• Entrez: 768
• UniProt ID: Q16790
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti G250 antibody, anti MN antibody, anti Carboni
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Carbonic Anhydrase 9 expression in Hela (A), 293T (B) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 49 kD)

Immunohistochemical analysis of Carbonic Anhydrase 9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunoprecipitation of Carbonic Anhydrase 9 from 0.5 mg Hela whole cell extract lysate, using Anti-Carbonic Anhydrase 9 Antibody Antibody.