CAPN5 antibody

• Catalog Number: orb341299
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CAPN5
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human Calpain 5
• Dilution range: WB: 1:500-1:2000, IF/ICC: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CAPN5
• Entrez: 12337, 171495, 726
• UniProt ID: O08688, Q8R4C0, O15484
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: NCL3; Calpain-5; Calpain htra-3; New calpain 3; nC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Calpain 5 expression in A431 (A), SKOV3 (B), mouse thymus (C), mouse brain (D) whole cell lysates. (Predicted band size: 73 kD; Observed band size: 73 kD)

Immunofluorescent analysis of Calpain 5 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.