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Catalog Number | orb1262036 |
---|---|
Category | Antibodies |
Description | CAPN3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Monkey |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This CAPN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 603-632 amino acids from the C-terminal region of human CAPN3. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 94 kDa |
Target | CAPN3 |
UniProt ID | P20807 |
NCBI | P20807 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Calpain-3, Calcium-activated neutral proteinase 3, Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of CAPN3 Antibody in HL-60 cell line lysates (35 ug/lane)
CAPN3 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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