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Catalog Number | orb1248221 |
---|---|
Category | Antibodies |
Description | CAPG Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Canine |
Reactivity | Human, Mouse, Porcine, Rat |
Immunogen | The immunogen for this antibody is: C-KAQVEIVTDGEEP |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 38kDa band observed in lysates of cell line U937 and of Human, Mouse and Rat Spleen (calculated MW of 38.5kDa according to NP_001738.2). In Peripheral Blood Lymphocytes, the observed band runs as ~40kDa. Recommended concentration: 0.1-0.3ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Approx 38kDa |
Target | CAPG |
UniProt ID | P40121 |
NCBI | NP_001738.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | CAPG, capping protein (actin filament), gelsolin-l Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 38kDa band observed in lysates of cell line U937 and of Human, Mouse and Rat Spleen (calculated MW of 38.5kDa according to NP_001738.2). In Peripheral Blood Lymphocytes, the observed band runs as ~40kDa. Recommended concentration: 0.1-0.3ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1248221 (0.3 ug/ml) staining of U937 (A) and Human Spleen (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-CAPG (0.3 ug/ml) staining of Periheral Blood Lymphocytes (orb1248220 lane A), orb1248221 lane B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1248221 (0.1 ug/ml) staining of Mouse (A) and Rat Spleen (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1248221 (0.3 ug/ml) staining of Pig Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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