Canine IL3 protein

• Catalog Number: orb80060
• Category: Proteins
• Description: IL3 Canine Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 14 kDa. The IL-3 is purified by proprietary chromatographic techniques.
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 97.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.
• Conjugation: Unconjugated
• Target: k9 IL 3
• Solubility (25°C): It is recommended to reconstitute the lyophilized IL3 in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: RPFSTDLPKQ YFTMINEIME MLNKSPSPSE EPLDSNEKET LLEDTLLRPN LDVFLNASSK FHKNGLLIWN NLKEFLPLLP TPTPRGEPIS IMENNWGDFQ RKLKKYLEAL DNFLNFKNKP.
• Biological Origin: E.coli
• Biological Activity: The ED50 as determined by the dose-dependant stimulation of TF-1 cells is 0.2 ng/ml, corresponding to Specific Activity of 5,000,000 IU/mg.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from concentrated (1mg/ml) solution in water containing 1X PBS pH7.4.
• Alternative names: MCGF (Mast cell growth factor) protein, Multi-CSF
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Canine IL3 protein