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Catalog Number | orb1946594 |
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Category | Assays and Kits |
Description | Canine CL(Cardiolipin) ELISA Kit |
Reactivity | Canine |
Range | 15.63-1000 ng/mL |
Target | CL |
Sensitivity | 1.5 ng/mL |
Alternative names | 1,3-Bis(sn-3-Phosphatidyl)-Sn-Glycerol Read more... |
Note | For research use only |
Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cardiolipin(CL) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cardiolipin(CL). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cardiolipin(CL) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Sample Types | tissue homogenates and other biological fluids |
Expiration Date | Please enquire. |
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