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Catalog Number | orb770517 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to CaMKII alpha/delta (phospho-Thr286) |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Mouse, Porcine, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human CaMK2 around the phosphorylation site of Thr286. AA range:256-305 |
Concentration | 1 mg/ml |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
Conjugation | Unconjugated |
MW | 54 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-CAMK2A antibody, anti-CAMKA antibody, anti-KI Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot analysis of HELA cells using Phospho-CaMKIIα/δ (T286) Polyclonal Antibody diluted at 1:500.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CaMK2 (Phospho-Thr286) Antibody.
Immunofluorescence analysis of COS7 cells, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.