CAMK2G (CAMK2 gamma) Antibody (C-term)

• Catalog Number: orb1939491
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB1246
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, WB: 1:500-1000, WB: 1:500, WB: 1:2000, IHC-P: 1:50~100, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 62607 Da
• Target: This CAMK2G (CAMK2 gamma) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-338 amino acids from the C-terminal region of human CAMK2G (CAMK2 gamma).
• UniProt ID: Q13555
• NCBI: NP_751913.1, NP_751909.1, NP_001213.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Calcium/calmodulin-dependent protein kinase type I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-CAMK2G (CAMK2 gamma) Antibody (C-term) at 1:2000 dilution. Lane 1: Jurkat whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CAMK2G (CAMK2 gamma) Antibody (C-term) at 1:500-1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: U-251 MG whole cell lysate. Lane 3: Human brain tissue lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-CAMK2G (CAMK2 gamma) Antibody (C-term) at 1:2000 dilution + human liver lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 63kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-CAMK2G (CAMK2 gamma) Antibody (C-term) at 1:500 dilution + T47D whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-CAMK2 gamma C-term Pab is used in Western blot to detect CAMK2 gamma in mouse heart tissue lysate.