Calreticulin Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb102221
• Category: Antibodies
• Description: Calreticulin Rabbit Polyclonal Antibody (FITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Calreticulin (101-200/417aa)
• Concentration: 1mg/ml
• Dilution range: IF=1:100-500
• Conjugation: FITC
• Target: CALR
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Autoantigen RO; CALR; CALR protein; CALR_HUMAN; cC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-Calreticulin antibody (orb102221), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-Calreticulin antibody, Dilution: 1 ug/Test, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.