CALR Antibody (Center)

• Catalog Number: orb1925305
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2143CT58.2.1
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1,κ
• Dilution range: WB: 1:1000, WB: 1:2000, WB: 1:500
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 48142 Da
• Target: This CALR antibody is generated from a mouse immunized with a recombinant protein from human CALR.
• UniProt ID: P27797
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Calreticulin, CRP55, Calregulin, Endoplasmic retic
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-CALR Antibody (Center) at dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CALR Antibody (Center) at dilution. Lane 1: HepG2 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: SH-SY5Y whole cell lysate. Lane 6: K562 whole cell lysate. Lane 7: NIH/3T3 whole cell lysate. Lane 8: C6 whole cell lysate. Lane 9: CHO whole cell lysat. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CALR Antibody (Center) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: HeLa whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: SH-SY5Y whole cell lysate. Lane 6: K562 whole cell lysate. Lane 7: NIH/3T3 whole cell lysate Lane 8: C6 whole cell lysate Lane 9: CHO whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.