Calponin 1 Antibody

• Catalog Number: orb749471
• Category: Antibodies
• Description: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CNN1/832
• Tested applications: IF, IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the anti-Calponin antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This anti-Calponin antibody is available for research use only.
• UniProt ID: P51911
• Storage: Store the anti-Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the anti-Calponin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with anti-Calponin antibody (clone CNN1/832).

IHC: Formalin-fixed, paraffin-embedded human uterus stained with anti-Calponin antibody (clone CNN1/832).

IHC: Formalin-fixed, paraffin-embedded rat uterus stained with anti-Calponin antibody (clone CNN1/832).

Immunofluorescent staining of PFA-fixed human K562 cells with anti-Calponin antibody (green, clone CNN1/832) and Reddot nuclear stain (red).

SDS-PAGE analysis of purified, BSA-free Calponin antibody (clone CNN1/832) as confirmation of integrity and purity.