Calponin 1 Antibody

• Catalog Number: orb749469
• Category: Antibodies
• Description: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34 kDa form and a 29kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CALP
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Crude human uterus extract was used as the immunogen for the Calponin antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Calponin antibody is available for research use only.
• UniProt ID: P51911
• Storage: Store the Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Calponin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
• Expiration Date: 12 months from date of receipt.

Western blot testing of human endometrium tissue lysate with Calponin antibody. Predicted molecular weight ~34 kDa.

SDS-PAGE Analysis of Purified, BSA-Free Calponin Antibody (clone CALP). Confirmation of Integrity and Purity of the Antibody.

IHC: Formalin-fixed, paraffin-embedded human uterus stained with Calponin antibody (CALP).

Immunofluorescent staining of PFA-fixed human K562 cells with Calponin antibody (green, clone CALP) and Reddot nuclear stain (red).

Flow cytometry testing of PFA-fixed human K562 cells with Calponin antibody (clone CALP); Red=isotype control, Blue=Calponin antibody.