Calponin 1 Antibody

• Catalog Number: orb606537
• Category: Antibodies
• Description: Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rCNN1/832
• Tested applications: FACS, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the recombinant Calponin antibody.
• Dilution range: Western blot: 0.5-1ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Calponin antibody is available for research use only.
• UniProt ID: P51911
• Storage: Store the recombinant Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Calponin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human uterus stained with recombinant Calponin antibody (clone rCNN1/832). Required HIER: boil tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Calponin antibody (clone rCNN1/832) as confirmation of integrity and purity.