Calponin 1 Antibody

• Catalog Number: orb386227
• Category: Antibodies
• Description: Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CNN1/1408R
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human, Rat
• Isotype: Rabbit IgG, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the recombinant Calponin antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Calponin antibody is available for research use only.
• UniProt ID: P51911
• Storage: Store the recombinant Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Calponin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human uterus stained with recombinant Calponin antibody (clone CNN1/1408R). Required HIER: steam sections in 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE rat uterus stained with recombinant Calponin antibody (clone CNN1/1408R). Required HIER: steam sections in 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of permeabilized human K562 cells with recombinant Calponin antibody (green, clone CNN1/1408R) and Reddot nuclear stain (red).

Flow cytometry testing of PFA-fixed human K562 cells with recombinant Calponin antibody (clone CNN1/1408R); Red=isotype control, Blue= recombinant Calponin antibody.

SDS-PAGE analysis of purified, BSA-free recombinant Calponin antibody (clone CNN1/1408R) as confirmation of integrity and purity.