Calponin 1 antibody

• Catalog Number: orb2276528
• Category: Antibodies
• Description: Calponin 1 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-455R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Immunogen: Synthetic peptide corresponding to residues on the C-terminus of human Calponin was used as an immunogen.
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P51911
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Calponin 1 basic smooth muscle; Calponin H1 smooth
• Note: For research use only
• Application notes: Positive Control: Colon: A moderate to strong Calponin 1 staining should be seen in the muscularis mucosae, muscularis propria and in the walls of larger blood vessels. Negative Control: Colon: Calponin 1 staining should be absent in all epithelial cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Calponin 1 immunostaining is regularly seen in myoepithelial cells of the breast.

A moderate intensity Calponin 1 immunostaining is seen in the muscularis mucosae in a colon sample.

A strong calponin 1 immunostaining occurs in the periductal smooth muscle layer in the epididymis.