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Calnexin Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb704311

DispatchUsually dispatched within 5-10 working days
$ 270.00
Catalog Numberorb704311
CategoryAntibodies
DescriptionCalnexin Recombinant Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityRecombinant
Clone Number24G6
Tested applicationsFC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityRat
ReactivityHuman, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Calnexin
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:50-200, IHC-F=1:400-800, ICC/IF=1:50, IF=1:100-500, Flow-Cyt=1:50
Form/AppearanceLiquid
ConjugationUnconjugated
MW65 kDa
TargetCANX
UniProt IDP27824
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesCalnexin-ER membrane marker; CANX; Cell adhesion m
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NoteFor research use only
Expiration Date12 months from date of receipt.
Calnexin Recombinant Rabbit Monoclonal Antibody

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Calnexin antibody (orb704311), dilution: 1:50, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Calnexin Recombinant Rabbit Monoclonal Antibody

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Calnexin) monoclonal Antibody, Unconjugated (orb704311) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Calnexin Recombinant Rabbit Monoclonal Antibody

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Calnexin) monoclonal Antibody, Unconjugated (orb704311) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Calnexin Recombinant Rabbit Monoclonal Antibody

PANC-1 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Calnexin) monoclonal Antibody, Unconjugated (orb704311) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Calnexin Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Calnexin) Monoclonal Antibody, Unconjugated (orb704311) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Calnexin Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Calnexin) Monoclonal Antibody, Unconjugated (orb704311) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Calnexin Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human pancreas tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Calnexin) Monoclonal Antibody, Unconjugated (orb704311) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Calnexin Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat heart tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Calnexin) Monoclonal Antibody, Unconjugated (orb704311) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Calnexin Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Calnexin) Monoclonal Antibody, Unconjugated (orb704311) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Calnexin Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Calnexin) Monoclonal Antibody, Unconjugated (orb704311) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Calnexin Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Hela cell lysate, Primary: Anti-Calnexin (orb704311) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 65 kD, Observed band size: 100 kD.

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    Human, Mouse, Rat

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    Rabbit

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    50 μl, 100 μl