Calnexin Antibody

• Catalog Number: orb385943
• Category: Antibodies
• Description: It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CANX/1541
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A partial human recombinant protein was used as the immunogen for the Calnexin antibody.
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Calnexin antibody is available for research use only.
• UniProt ID: P27824
• Storage: Store the Calnexin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the Calnexin antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human small intestine carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min.

IHC testing of FFPE human renal cell carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min.

IHC testing of FFPE human renal cell carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min.

Western blot testing of human kidney lysate with Calnexin antibody (clone CANX/1541). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.

Western blot testing of human 1) HeLa and 2) MCF7 cell lysate with Calnexin antibody (clone CANX/1541). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.

Immunofluorescent staining of human HeLa cells with Calnexin antibody (green) (clone CANX/1541). RedDot used as nuclear counterstain (red).

SDS-PAGE analysis of purified, BSA-free Calnexin antibody (clone CANX/1541) as confirmation of integrity and purity.