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Catalog Number | orb371656 |
---|---|
Category | Antibodies |
Description | Caldesmon/CALD1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human Caldesmon recombinant protein (Position: M1-E120). Human Caldesmon shares 87.6% amino acid (aa) sequence identity with rat Caldesmon. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 93231 MW |
UniProt ID | Q05682 |
Sensitivity | > 5000 cells |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Caldesmon;CDM;CALD1;CAD, CDM; Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of Caldesmon using anti-Caldesmon antibody.Lane 1:human U20S cell; 2:human A549 cell; 3:human HepG2 cell; 4:human HeLa cell; 5:human CACO-2 cell; 6:mouse NIH/3T3 cell.
IHC analysis of Caldesmon using anti-Caldesmon antibody. Caldesmon was detected in a paraffin-embedded section of human intestinal cancer tissue.
IHC analysis of Caldesmon using anti-Caldesmon antibody. Caldesmon was detected in a paraffin-embedded section of rat intestine tissue.
IHC analysis of Caldesmon using anti-Caldesmon antibody. Caldesmon was detected in a paraffin-embedded section of mouse intestine tissue.
IHC, WB | |
Human, Mouse, Rabbit | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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