Caldesmon / CAD Antibody HMW

• Catalog Number: orb385973
• Category: Antibodies
• Description: Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: A partial human protein was used as the immunogen for the Caldesmon antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Caldesmon antibody is available for research use only.
• UniProt ID: Q05682
• Storage: Store the Caldesmon antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the Caldesmon antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human uterus with recombinant Caldesmon antibody. Required HIER: boil tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min.

IHC testing of FFPE rat uterus with recombinant Caldesmon antibody. Required HIER: boil tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min.

Western blot testing of human ovary tissue lysate using Caldesmon antibody. Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).