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Catalog Number | orb749859 |
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Category | Antibodies |
Description | Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CALD1/820 + h-CALD |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant full-length human protein (CALD1/820) and crude human uterus extract (h-CALD) were used as the immunogen for the CAD antibody cocktail. |
Dilution range | Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This CAD antibody cocktail is available for research use only. |
UniProt ID | Q05682 |
Storage | Store the CAD antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the CAD antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC: Formalin-fixed, paraffin-embedded human uterus stained with CAD antibody (CALD1/820 + h-CALD).
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